The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation
CRYOBIOLOGY, vol.58, no.2, pp.134-138, 2009 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 58 Issue: 2
- Publication Date: 2009
- Doi Number: 10.1016/j.cryobiol.2008.11.006
- Journal Name: CRYOBIOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.134-138
- Keywords: Bull semen freezing, Artificial insemination, Oxidative stress parameters, Antioxidants
- Erciyes University Affiliated: Yes
Abstract
Oxidative stress significantly damages sperm functions such as motility, functional integrity, endogenous antioxidant enzyme activities and fertility due to lipid peroxidation induced by reactive oxygen species (ROS). The aim of this study was to determine the effects of antioxidants such as taurine and cysteine in Bioxcell (R) extender on standard semen parameters, fertilizing ability, lipid peroxidation (LPO) and antioxidant activities comprising reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) after the cryopreservation/thawing of bull semen. Nine ejaculates for each bull were included in the study. Three groups, namely taurine (2 mM), cysteine (2 mM), and control, were designed to analyze the antioxidants in Bioxcell (R). Insemination doses were processed so that each 0.25-ml straw contained 15 x 10(6) sperm.