© 2021 Elsevier B.V.The importance of magnetic nanomaterials and magnetic hybrid materials, which are classified as new generation materials, in analytical applications is increasingly understood, and research on the adaptation of these materials to analytical methods has gained momentum. Development of sample preparation techniques and sensor systems using magnetic nanomaterials for the analysis of inorganic, organic and biomolecules in biological samples, which are among the samples that analytical chemists work on most, are among the priority issues. Therefore in this review, we focused on the use of magnetic nanomaterials for the bioanalytical applications including inorganic and organic species and biomolecules in different biological samples such as primarily blood, serum, plasma, tissue extracts, urine and milk. We summarized recent progresses, prevailing techniques, applied formats, and future trends in sample preparation-analysis methods and sensors based on magnetic nanomaterials (Mag-NMs). First, we provided a brief introduction of magnetic nanomaterials, especially their magnetic properties that can be utilized for bioanalytical applications. Second, we discussed the synthesis of these Mag-NMs. Third, we reviewed recent advances in bioanalytical applications of the Mag-NMs in different formats. Finally, recently literature studies on the relevance of Mag-NMs for bioanalysis applications were presented.