Identification of the new Pseudomonas species for aquaculture and important biochemical characteristics


Duman M., Satıcıoğlu İ. B. , Altun S.

19th International Conference on Diseases of Fish and Shellfish, Porto, Portekiz, 9 - 12 Eylül 2019, ss.329

  • Basıldığı Şehir: Porto
  • Basıldığı Ülke: Portekiz
  • Sayfa Sayıları: ss.329

Özet

Introduction: The genus Pseudomonas is one of the most complex Gram-negative bacterial

genera as it currently is the genus with the largest number of species. While Pseudomonas cause

severe disease for humans, the incidence or transmission route not known very well from aquaculture

to humans. Recently, there are many possible novel Pseudomonas species identified,

but a few of them identified from fish. In the present study, the identification of Pseudomonas

species recovered from farmed salmonid species and the determination of the most important

biochemical characteristics were aimed.

Methodology: Pseudomonas species were isolated from farmed salmonids in Turkey, 2013 -

2018. The strains were identified by 16S rRNA gene sequence analysis with the similarity index

on NCBI database. Growth condition of Pseudomonas spp. on different salinity, incubation

temperature, distilled and tap water were showed by using Tryptic soy broth media. In addition,

the fluorescent pigment and biofilm production were determined by using Pseudomonas selective

agar and broth media.

Results: The Pseudomonas species isolated from rainbow trout (Onchorhynchus mykiss), local

trout species (Salvelinus fontinalis, Salmo trutta magrostigma, Salmo trutta labrax), Carp

(Cyrpinus carpio) and also farm water samples. More than ten different Pseudomonas species

were identified with the similarity of NCBI database such as P. jessenii, P. madelii, P. gessardii

and a number of them are possible for novel species. The most of our isolates could growth on

0 - 6% NaCl-TSB medium, distilled and tap water and also 4 - 37 °C incubation temperature.

Conclusion: This is the first comprehensive research about Pseudomonas species determination

on aquaculture in Turkey. The identified species were determined in the first time in salmonids

and also some of them are possible novel Pseudomonas species isolated from aquaculture. Also

our findings showed that the Pseudomonas can growth on distilled and tap water in addition

broad range of salinity and incubation temperature. Thus, the transmission of this agent from

aquaculture to human/animal/environment via water pose a big risk factor.

Keywords: Pseudomonas, new species, biochemical characteristics

Funding: Scientific and Technological Research Council (TUBITAK) of Turkey, project number:

118O420.