A randomized controlled study investigated the effects of ultrasound and laser treatments on wound healing in rats. The duration of the inflammatory phase decreased with both laser and ultrasound treatments; however, laser was more effective than ultrasound, with more significant results. The proliferation phase showed, for both treatments, an increase in the level of hydroxyproline and the number of fibroblasts, as well as stimulation of the collagen synthesis and the composition. Laser treatment was again more effective than ultrasound. The wound breaking strength was significantly higher with both treatments, and no statistically significant difference emerged between the laser and ultrasound groups, although laser treatment provided a much greater increase in the wound breaking strength than ultrasound. Both treatments have beneficial effects on the inflammatory, proliferation, and maturation phases of wound healing. Both can be used successfully for decubitis ulcers and chronic wounds, in conjunction with conventional therapies such as debridement and daily wound caring. However, laser treatment was more effective than ultrasound in the first two phases of wound healing.