In-silico identification of microRNAs potentially targeting the PGC1? gene that regulates bovine mitochondrial biogenesis


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Prastowo S., Widyas N., Ratriyanto A., Sohel M. M. H.

2nd UGM International Conference on Tropical Agriculture, Yogyakarta, Indonesia, 26 - 27 October 2017, vol.1, no.1, pp.1-5

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Yogyakarta
  • Country: Indonesia
  • Page Numbers: pp.1-5
  • Erciyes University Affiliated: Yes

Abstract

Post-transcriptional gene regulation in eukaryotic cells is mediated through several molecules including noncoding RNAs. Among the noncoding RNAs, microRNAs (miRNAs) are the master regulator of gene expression and therefore control various biological processes in living cells. Mitochondrial biogenesis is a biological process by which cell increases the number of the active mitochondrion to produce more ATP. The presence of peroxisome proliferator-activated receptor γ coactivator-1alpha (PGC1α) protein determines mitochondrial biogenesis to enhance cellular energy production in cells. Using in-silicoapproach, this study aims to identify the miRNAs that could potentially target PGC1α gene to regulate mitochondrial biogenesis. For this, four miRNA target prediction databases were employed to identify the potential miRNAs which may target PGC1α gene. The prediction results showed that a total of 27, 47, 77 and 79 miRNAs were predicted by MirTarget2, PicTar, TargetScan, and miRanda, respectively. Following the clustering process, we found seven miRNAs were identified as potential candidates by all four databases. Out of seven miRNAs, three miRNAs namely miR-323, miR-222, and miR-137 were found to have 100% sequence similarity between human (hsa) and bovine (bta) miRNAs. miRNA binding site analysis showed that miR-323, miR-222, and miR-137 have five, two and three seeding regions, respectively, in the PGC1α 3’UTR. Our study identified three miRNAs that have multiple binding sites at the PGC1α 3’UTR and could potentially modulate mitochondrial biogenesis. However, a validation study experiment needs to be performed to validate the interaction of these miRNAs with PGC1α gene.