Comparison of Etest with the broth microdilution method in susceptibility testing of yeast isolates against four antifungals

Koc A. N., Gokahmetoglu S., Oğuzkaya M.

MYCOSES, vol.43, pp.293-297, 2000 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 43
  • Publication Date: 2000
  • Doi Number: 10.1046/j.1439-0507.2000.00574.x
  • Journal Name: MYCOSES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.293-297
  • Keywords: yeasts, susceptibility testing, Etest, microdilution, amphotericin B, fluconazole, itraconazole, ketoconazole, MACRODILUTION, FLUCONAZOLE
  • Erciyes University Affiliated: Yes

Abstract

A comparative evaluation of the Etest and the broth microdilution methods for antifungal susceptibility testing of 102 clinical yeast isolates against amphotericin B, fluconazole, itraconazole, and ketoconazole was conducted. The agreements between the Etest and the broth microdilution methods were 93.1% for amphotericin B 85.2% for ketoconazole, 82.3% for itraconazole and 79.4% for fluconazole. These results suggest that the Etest approach to antifungal susceptibility testing may be a viable alternative to the NCCLS reference methods for testing yeasts, but that further evaluations are needed.