Akademik Veri Yönetim Sistemi
XXVII. Balkan Clinical Laboratory Federation Meeting BCLF 2019 XXX. National Congress of the Turkish Biochemical Society TBS 2019, Antalya, Türkiye, 27 - 31 Ekim 2019, cilt.44, sa.13, ss.43
O-013 The Effect of Hemolysis and Storage Conditions on Insulin Stability
Didem Barlak Keti̇, Sabahattin Muhtaroglu
Department of Medical Biochemistry, Erciyes University, Kayseri, Turkey
OBJECTIVES:Biochemical or spectrophotometric measurements are known to be more affected by hemolysis when compared to immunochemical analysis. This situation can often lead to less consideration on immunochemical assays. Threshold values at which hemolysis affects immunochemical tests are indicated in our kit inserts, but there is no value related to insulin. Therefore, the aim of this study is to determine the hemolysis threshold for insulin and the effect of storage conditions on serum insulin stability.
MATERIALS and METHODS:Serum pools were formed from the samples of the routine laboratory. Serum samples of equal volume were transferred to seven tubes. The tubes were designed as only serum in the first tube, serum + assay diluent in the second tube, and serum + hemolysate in the 3-7 tubes which correspond to 50,100, 200, 400 and 800, respectively hemolysis index. In addition, insulin levels were measured in the patient samples with <20 (n = 10), 20-50 (n=10), 50-100 (n=10) and 100-200 (n=10) hemolysis index immediately and after 8 hours at room temperature. RESULTS:Negative bias was detected as 10% in the samples with below 200 mg/dL hemolysis index which were analysed immediately after centrifugation. Negative bias was determined as <10%, 27.6% and 29.5% in the samples <20, 20-50 and 50-100 hemolysis index, respectively which stayed for 8 hours at room temperature.
CONCLUSIONS:Hemolysis index should be considered when reporting insulin levels. Insulin analysis is not suitable for hemolysed serum samples that have waited 8 hours at room temperature.
Keywords: Hemolysis, insulin, stability