A new method has been developed based on UV-Vis spectrophotometer measurement after liquid-phase microextraction using deep eutectic solvents for the determination of allura red dye. Tetra-n-butyl ammonium bromide and decanoic acid were used as deep eutectic solvent components by the developed method. The effects of the method on recovery, mainly pH, deep eutectic solvent components and volume, tetrahydrofuran volume, matrix effects, model solution volume, ultrasonication time and centrifugation time were also investigated. Allura red was extracted into the deep eutectic solvent phase at pH 4.0. The relative standard deviations were lower than 5%. The limit of detection (LOD) and preconcentration factor were found to be 3.92 mu g/L and 13, respectively. The accuracy of the method was confirmed by the addition method on water, detergent and chocolate samples.