Akademik Veri Yönetim Sistemi
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, cilt.28, sa.3, 2025 (SCI-Expanded, Scopus)
This study characterized the venom of the Algerian honeybee, Apis mellifera intermissa, and evaluated its key biological activities. Biochemical analysis by High-performance liquid chromatography (HPLC) analysis revealed that melittin, the main component of bee venom, constituted approximately 38 % of its dry weight. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed the presence of major components, including melittin (similar to 3-5 kDa), phospholipase A(2) (similar to 15-20 kDa), and hyaluronidase (similar to 44 kDa). The venom exhibited strong antioxidant activity, as demonstrated by Ferric Reducing Antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Potent, broad-spectrum antibacterial activity was observed against Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhimurium, Enterococcus faecalis, and Acinetobacter baumannii, yielding low minimum inhibitory and bactericidal concentrations (MIC/MBC). The anti-inflammatory potential was evidenced by the inhibition of bovine serum albumin (BSA) denaturation. Furthermore, the venom exhibited significant cytotoxicity against MCF-7 and MDA-MB-231 human breast cancer cell lines, with half-maximal inhibitory concentrations (IC50) of 17.17 mu g/mL and 11.90 mu g/mL, respectively. These findings highlight venom as a rich source of bioactive compounds with multifaceted therapeutic potential for pharmacological development.