The exhaustion characteristic features of CI reactive red 194dye on cotton fabricunder differentdyeing conditions wereinvestigated in this study. For this purpose, dye temperature, time intervals, the concentration of dye, salt and soda were selected as dyeing inputs. Dye solutionsamples weredrawn from the dye-bathfrom the beginning to the end of the process at 10 min intervals for determining dye exhaustion. The transmittance of the colored dye bath sample was measured by using the UV-Vis technique. The dyeing results were investigated by statistical analysisusing ANOVA and regression curves. In addition, the times of half dyeing (t(1/2)) were evaluated. The maximum exhaustion conditions were obtained and discussed according to the statistical outputs.