Today, several fruit species are used for different purposes in alternative medicine. Among those species, barberry species are commonly used in treatment of various diseases. Jeti-Oguz District of Issyk Kul located around Issyk Lake in North-east of Kyrgyzstan has a unique flora and wild Berberis sphaerocarpa Kar. et Kit. (spherical-fruited barberry) species have a great place in regional flora. The fruits, leaves and roots of these species are commonly used by local people as functional products in alternative medicine. The most significant parameter in this wild population is the morphological variation in fruits and leaves. In this study, pomological analyses were performed on ripened black fruits of 26 wild genotypes. Pomological analyses yielded the fruit weights as between 0.23 g (01-JO-006) and 0.61 g (01-JO-025); total soluble solids as between 16.67% (01-JO-014) and 18.73% (01-JO-010 and 01-JO-018). Fruit shapes of genotypes were identified as long, long-spherical, spherical and oblate spherical. Significant variations were observed in morphological characteristics of spherical-fruited barberry genotypes through pomological analyses and field observations.