Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor


Nisari M., Kaymak E., Ertekin T., Kaan D. , İnanç N., Özdamar S.

Diğer, ss.316-321, 2019

  • Basım Tarihi: 2019
  • Sayfa Sayıları: ss.316-321

Özet

Objectives: Cancer is the second most common cause of death in the world. Several chemotherapeutic drugs have been studied for their anticancer features. Paclitaxel is one of these chemotherapeutic drugs with high medicinal interest. This study was conducted in order to investigate effects of paclitaxel on lipid peroxidation and antioxidant enzymes in tissues of mice bearing solid-form Ehrlich tumors. Methods: In this study, 36 Balb/C male mice aged 8-10 weeks and weighing 25-30 g were used. Six mice were kept as cancer stock to produce Ehrlich Ascites Tumor (EAT) cells. Thirty mice were distributed into three groups as healthy control, tumor control and paclitaxel treatment. 0.1 ml physiologic saline solution was administered into mice in healthy control subcutaneously (s.c.) for 15 days. The animals in tumor control and Paclitaxel treatment groups received 1x106 EAT cells s.c. through nape skin on the first day of the experiment. After the application of EAT cells, 10 mg/kg Paclitaxel was injected intraperitoneally on days 4, 9 and 14. At the end of the study (on day 15), animals were sacrificed and liver, kidney, brain and testis tissues were excised and analyzed for Malondialdehyde (MDA), by using TBARS method, superoxide dismutase (SOD) and catalase (CAT) activities spectrophotometrically. Results: Compared to the results of healthy control group, tumor increased kidney and liver MDA levels development slightly but not significantly. Paclitaxel treatment significantly reduced the increased MDA levels in kidney and liver (p<0.001). Paclitaxel had no effect on testis MDA but brain MDA level reduced with the help of EAT cell injection and Paclitaxel returned the brain MDA level close to the level of healthy control (p<0.001). EAT cell injection reduced catalase activity in kidney and liver (p<0.001) and Paclitaxel had no effect on catalase activities in these tissues. In EAT cell injected mice; testis and brain catalase activities were higher than that of healthy control group that were returned to control levels by Paclitaxel treatment. Paclitaxel had no significant effect on decreased kidney and liver SOD activities whereas it significantly reduced the increased SOD activities in testis (p<0.05) and in brain (p<0.01). Conclusion: Paclitaxel alleviated the lipid peroxidation in kidney and liver but had no effect on antioxidant status in these tissues of solid-Ehrlich tumor-bearing mice. Keywords: Antioxidant enzymes, lipid peroxidation, paclitaxel, solid-form Ehrlich tumor