Studies on the Effectiveness of Ozone Therapy on the Treatment of Experimentally Induced Keratitis with Candida albicans in Rabbits

Creative Commons License

Varol K., KOÇ A. N., ÇAKIR BAYRAM L., ARDA H., KELEŞ İ., ÜNLÜ M., ...More

Seminars in Ophthalmology, vol.37, no.2, pp.253-264, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 2
  • Publication Date: 2022
  • Doi Number: 10.1080/08820538.2021.1995006
  • Journal Name: Seminars in Ophthalmology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CINAHL, EMBASE, MEDLINE
  • Page Numbers: pp.253-264
  • Keywords: Ozone, Ozonated olive oil, Ozonated distilled water, Keratitis, Experimental keratitis, Fungal keratitis, Candida albicans, FUNGAL KERATITIS, TOPICAL FLUCONAZOLE, OIL
  • Erciyes University Affiliated: Yes


© 2021 Taylor & Francis.Purpose: In the present study, antifungal activity of ozonated olive oil (OZO) and ozonated distilled water (ODW) in the treatment of experimentally induced keratitis with C. albicans in rabbits were investigated. Methods: The Groups were composed of as 1, 2, 3, 4, 5, 7 (n = 5 rabbits, 10 eyes/in each group) and Group 6 (n = 10 rabbits, 20 eyes/in the group). Fourty-eight hours after C. albicans inoculation; Group 1 received fluconazle (FLU)+OZO drops, Group 2 received FLU drop, Group 3 received OZO drop, Group 4 received FLU+ODW drops, Group 5 received ODW drop, Group 6 (infected control group) and Group 7 received PBS drop (negative control group). Treatment continued in all groups for 22 days for every 8 hours. Results: Cornea cultures made 24 days post inoculation revealed statistically significant differences (p < 0,05) with concern to C. albicans amounts between Group 6 and Group 1–5. Statistical comparison of corneal opacity and corneal ulcer and conjunctivitis values among the Group 6 and Group 1–5 were also different significantly (p < 0,05) on days 20 and 24 post inoculation. Conclusion: OZO and ODW were found to be effective in treating C. albicans keratitis in the present study. It has also been proven by this study that ODW contain 26 μg/ml was the most effective in the treatment of C. albicans keratitis.