Studies on the Effectiveness of Ozone Therapy on the Treatment of Experimentally Induced Keratitis with Candida albicans in Rabbits


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Varol K., KOÇ A. N., ÇAKIR BAYRAM L., ARDA H., KELEŞ İ., ÜNLÜ M., ...More

Seminars in Ophthalmology, vol.37, no.2, pp.253-264, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 2
  • Publication Date: 2022
  • Doi Number: 10.1080/08820538.2021.1995006
  • Journal Name: Seminars in Ophthalmology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CINAHL, EMBASE, MEDLINE
  • Page Numbers: pp.253-264
  • Keywords: Ozone, Ozonated olive oil, Ozonated distilled water, Keratitis, Experimental keratitis, Fungal keratitis, Candida albicans, FUNGAL KERATITIS, TOPICAL FLUCONAZOLE, OIL
  • Erciyes University Affiliated: Yes

Abstract

© 2021 Taylor & Francis.Purpose: In the present study, antifungal activity of ozonated olive oil (OZO) and ozonated distilled water (ODW) in the treatment of experimentally induced keratitis with C. albicans in rabbits were investigated. Methods: The Groups were composed of as 1, 2, 3, 4, 5, 7 (n = 5 rabbits, 10 eyes/in each group) and Group 6 (n = 10 rabbits, 20 eyes/in the group). Fourty-eight hours after C. albicans inoculation; Group 1 received fluconazle (FLU)+OZO drops, Group 2 received FLU drop, Group 3 received OZO drop, Group 4 received FLU+ODW drops, Group 5 received ODW drop, Group 6 (infected control group) and Group 7 received PBS drop (negative control group). Treatment continued in all groups for 22 days for every 8 hours. Results: Cornea cultures made 24 days post inoculation revealed statistically significant differences (p < 0,05) with concern to C. albicans amounts between Group 6 and Group 1–5. Statistical comparison of corneal opacity and corneal ulcer and conjunctivitis values among the Group 6 and Group 1–5 were also different significantly (p < 0,05) on days 20 and 24 post inoculation. Conclusion: OZO and ODW were found to be effective in treating C. albicans keratitis in the present study. It has also been proven by this study that ODW contain 26 μg/ml was the most effective in the treatment of C. albicans keratitis.