SAĞLIKLI SIĞIRLARIN DIŞKILARINDAN LİSTERİA SPP. İZOLASYON VE İDENTİFİKASYONU* Isolation and Identification of Listeria Spp. from Faeces Samples of Healty Cattle


Sağlık Bilimleri Dergisi (Journal of Health Sciences), vol.14, no.3, pp.191-197, 2005 (Peer-Reviewed Journal)


In this study, Listeria spp were isolated

and identificated in faecal samples from 100 healthy

cows in a Private Commercial Dairy Products

Company in different periods (January, October,

April, July) of the 2003. For the isolation of Listeria

spp., Listeria Selective Broth (Oxoid) and Listeria

Selective Agar (Oxoid) were used on the samples of

faeces. Upon the first isolation of the samples, the

method of cold enrichment was used for the samples

on which isolation was not possible. Then the

isolation procedure was reapplied to negative

samples after the third and the seventh week. In order

to grow isolates common biochemistry tests were

applied and API Listeria (Biomerieux, France) Test

Kit was used. A hundred faeces samples were taken

from healthy cattle in the months of January, October,

April and July. In the first isolation studies carried

out in October, out of 100 faeces, 52 (%52), in

January 55 (%55), in April, 31 (%31) and in July 30

(%30) were positive for Listeria spp. In the cold

enrichment process, no production of Listeria spp was

observed in the 3


rd week. In the seventh week, seven

(%14.58) of 48 negative samples in October, six (%

13.31) of 45 negative samples in January, seven (%

11.59) of 69 samples in April, six (%8.57) of 70

negative samples in July were isolated Listeria spp.

According to the findings in our research, the

spreading, to the nature in the cow faeces of Listeria

spp. which has a wide range of survival temperature,

is a potantial danger for the health of humans and