Research Information System
Sağlık Bilimleri Dergisi (Journal of Health Sciences), vol.14, no.3, pp.191-197, 2005 (Peer-Reviewed Journal)
In this study, Listeria spp were isolated
and identificated in faecal samples from 100 healthy
cows in a Private Commercial Dairy Products
Company in different periods (January, October,
April, July) of the 2003. For the isolation of Listeria
spp., Listeria Selective Broth (Oxoid) and Listeria
Selective Agar (Oxoid) were used on the samples of
faeces. Upon the first isolation of the samples, the
method of cold enrichment was used for the samples
on which isolation was not possible. Then the
isolation procedure was reapplied to negative
samples after the third and the seventh week. In order
to grow isolates common biochemistry tests were
applied and API Listeria (Biomerieux, France) Test
Kit was used. A hundred faeces samples were taken
from healthy cattle in the months of January, October,
April and July. In the first isolation studies carried
out in October, out of 100 faeces, 52 (%52), in
January 55 (%55), in April, 31 (%31) and in July 30
(%30) were positive for Listeria spp. In the cold
enrichment process, no production of Listeria spp was
observed in the 3
rd week. In the seventh week, seven
(%14.58) of 48 negative samples in October, six (%
13.31) of 45 negative samples in January, seven (%
11.59) of 69 samples in April, six (%8.57) of 70
negative samples in July were isolated Listeria spp.
According to the findings in our research, the
spreading, to the nature in the cow faeces of Listeria
spp. which has a wide range of survival temperature,
is a potantial danger for the health of humans and
animals.