Akademik Veri Yönetim Sistemi
Genetic Resources and Crop Evolution, cilt.72, sa.7, ss.9001-9019, 2025 (SCI-Expanded, Scopus)
Molecular marker breeding is a powerful tool for screening targeted genetic material, evaluating genetic differences and targeting a crop improvement potential. Hence, DNAs of leaf samples belonging to 87 Rubus genotypes from different regions of Türkiye were isolated with the application marking system. Polymerase chain reaction (PCR) products were obtained with 16 inter simple sequence repeat (ISSR) primers and band lengths ranging from 150 bp [(AGC)6G, (AG)7YC, VHVG(TG)7] to 1500 bp [HVH(CA)7 T] were detected. A total of 293 bands were obtained in terms of genetic diversity and 292 were shown to be polymorphic and 99.75% polymorphism rate. When the obtained results were evaluated, it was confirmed that there was a significant genetic diversity in the genetic material. In the molecularly presented dendrogram, the similarity level between genetic materials can be determined between 0.68 and 1, the average similarity coefficient was determined as 0.84 and two main groups (A and B) were established. Almost all of the genotypes are genetically separated from each other. Rubus cultivars and genotypes were divided into five populations using a model-based structure algorithm. Based on the STRUCTURE analysis, individuals in the second, fourth, and fifth subpopulations were identified as pure cultivars and are expected to contribute to hybridization research. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis and principal component analysis (PCA) also supported these five different populations and confirmed the reliability of the STRUCTURE analysis. According to the obtained data, the low number of populations was due to the high gene flow rate between the regions where the samples were taken. The study is the first in this field with blackberry materials representing different regions of Türkiye. The genetic diversity observed in the Rubus genetic material reveals that this material is a valuable genetic resource for comprehensive breeding programs. In addition, these cultivars and genotypes are considered as promising candidates for breeding aimed at increasing blackberry production.