Cytogenetic analysis of peripheral blood lymphocytes of hospital staff occupationally exposed to low doses of ionizing radiation


EKEN A., Aydın A., Erdem O., Akay C., Sanal H. T., Soykut B., ...Daha Fazla

TOXICOLOGY AND INDUSTRIAL HEALTH, cilt.26, sa.5, ss.273-280, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 5
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1177/0748233710365693
  • Dergi Adı: TOXICOLOGY AND INDUSTRIAL HEALTH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.273-280
  • Anahtar Kelimeler: ionizing radiation, occupational exposure, micronuclei, sister chromatid exchange, peripheral lymphocytes, SISTER-CHROMATID EXCHANGES, HUMAN MICRONUCLEUS PROJECT, INDUCED GENOMIC INSTABILITY, DNA-DAMAGE, CHROMOSOMAL-ABERRATIONS, COMET ASSAY, OXIDATIVE STRESS, FEMALE MICE, LOW-LEVEL, WORKERS
  • Erciyes Üniversitesi Adresli: Hayır

Özet

Ionizing radiation is known to induce mutations and cell transformations, predominantly by causing single-strand and double-strand DNA breakage, thereby leading to chromosome instability and carcinogenesis. The aim of this study was to evaluate genotoxic effects in hospital staff exposed to low-dose ionizing radiation in comparison with a selected control group, by using the cytokinesis-blocked micronucleus (CBMN) and sister chromatid exchange (SCE) tests in peripheral blood lymphocytes. The study included 40 exposed radiology staff and 30 control subjects. The frequency of micronuclei (MN) was significantly increased in radiation-exposed groups compared with control persons (p < 0.05). The frequency of SCE did not show any significant difference in the exposed individuals in comparison to the controls. Our results showed that low-level chronic occupational exposure to ionizing radiation causes an increase of MN frequency in chromosomes, even though the absorbed doses were below the permissible limits. Our studies indicate that the CBMN assay is considered to be sensitive test in contrast to SCE analysis to evaluate chromosomal damage induced by ionizing radiation.