Comparison of ELISA and flow cytometry for measurement of interleukin-1 beta, interleukin-6 and tumor necrosis factor-alpha

ÇETİN A., Sen A., Cetin I., ÇİMEN B., ÇİMEN L., Savas G., ...Daha Fazla

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.43, sa.5, ss.540-548, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 5
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1515/tjb-2017-0164
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.540-548
  • Anahtar Kelimeler: Method comparison, Flow cytometry, ELISA, IL-1 beta, IL-6, TNF-alpha, HUMAN CYTOKINES, BEAD ARRAY, ASSAYS, SERUM, QUANTITATION, TECHNOLOGY, BIOMARKERS, GUIDELINES, REGRESSION
  • Erciyes Üniversitesi Adresli: Evet

Özet

Background: Although majority of the previous studies have shown a good correlation between enzyme linked immuno sorbent assay (ELISA) and flow cytometry in terms of cytokines, two laboratory methods usually were compared with the regression analysis and correlation in the literature. This study aimed at comparing the ELISA and flow cytometry assay for measuring cytokines by using two different statistical methods, regression analysis and Bland-Altman plot.