Expression of Vascular Endothelial Growth Factor Its Receptors flk1 fms flk1 KDR flt4 and Vascular Endothelial Growth Inhibitor in Lungs of Japanese quail Coturnix coturnix japonica during the Post Hatching Period

Sağsöz H., Liman N. , Alan E.

30th European Association of Veterinary Anatomists Congress, Cluj-Napoca, Romania, 23 - 26 July 2014, vol.43, pp.81-82

  • Publication Type: Conference Paper / Summary Text
  • Volume: 43
  • City: Cluj-Napoca
  • Country: Romania
  • Page Numbers: pp.81-82


Introduction: Vascular endothelial growth factor (VEGF)

and its receptors VEGFR1-Flt1, VEGFR2-KDR/Flk1 and

VEGFR3/Flt4 play an im portant role in vasculogenesis

and physiological angiogenesis. The lung actions of VEGF

are overarching since they affect lung development and

structural maintenance of the adult lung. To determine

the expression pattern of VEGF and its receptors in the

lung, we analyzed levels of VEGF, VEGFR1-Flt1, VEGFR2-

KDR/Flk1 and VEGFR3/Flt4 and Vascular endothelial

growth inhibitor (VEGI) in lung of quail during posthatching


Methods: Thirty Japanese quails were obtained from the

Safiye Cikrikcioglu Vocational College, Erciyes University

(Kayseri). Five quails each, aged 1, 5, 10, 15, 30, and

125 days, were sacrified under ether anaesthesia and their

lungs were quickly removed for immunohistochemical

analysis. The tissues were fixed in 10%neutral formalin

solution for 24 h at room temperature.

Results: Immunohistochemical studies revealed the presence

of cytoplasmic and membrane staining for VEGI,

VEGF and its receptors were observed in bronchi, atria

and air capillaries as well as blood vessels in the lung

throughout the post-hatching period. Immunoreactivity

for Flt1/fms and Flk1/KDR in the bronchi, atria and air

capillaries were stronger than those of VEGF, VEGI and

flt4, particularly from hatching to the 15th days posthatching.

Besides, immunoreactivity for VEGF in the atria

and air capillaries was strong on the 30th and 125th days

of post-hatching period. However, VEGI immunoreaction

was relatively similar in the all parts of the lung during

post-hatching period.

Conclusions: We conclude that post-hatching period may

be precisely regulated by the combined functions like

growth and vasculogenesis/angiogenesis of VEGF family

members, angiogenic VEGF and VEGF receptors, and the

angiogenesis inhibitor VEGI in the guail lung,.

This study was approved by the Ethics Committee of

Erciyes University.