Detection of Pasteurella multocida, Mannhemia haemolytica, Histophilus somni and Mycoplasma bovis in cattle lung

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CENGİZ S., ADIGÜZEL M. C., DİNÇ G.

REVISTA MEXICANA DE CIENCIAS PECUARIAS, cilt.12, sa.3, ss.710-720, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 3
  • Basım Tarihi: 2021
  • Doi Numarası: 10.22319/rmcp.v12i3.5469
  • Dergi Adı: REVISTA MEXICANA DE CIENCIAS PECUARIAS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Fuente Academica Plus, EMBASE, Directory of Open Access Journals, DIALNET
  • Sayfa Sayıları: ss.710-720
  • Anahtar Kelimeler: Cattle, Molecular analysis, Lung, Respiratory disease, HAEMOPHILUS-SOMNUS, MANNHEIMIA-HAEMOLYTICA, RESPIRATORY-DISEASE, PCR, NASAL, IDENTIFICATION, VACCINATION, INFECTIONS, DIAGNOSIS, HEALTHY
  • Erciyes Üniversitesi Adresli: Evet

Özet

In this study, it was aimed to determine of P. multocida, M. haemolytica, H. somni and M. bovis in macroscopically healthy cattle lungs by PCR. The study was carried out on 82 macroscopically healthy cattle lung. DNA extraction was performed to the lung samples. PCR was then performed using all specific primers. By molecular evaluation, positive results were achieved for P. multocida, M. haemolytica, H. somni and M. bovis in 4 (4.8 %), 4 (4.8 %), 6 (7.3 %) and 3 (3.6 %) of the samples, respectively. Mix infections were detected in five samples. Of the samples, two were positive for both P. multocida and M. haemolytica, two were positive for both M. haemolytica and H. somni and one was positive for both P. multocida and H. somni. However, a positive sample, which carried all of pathogens, was not detected. In conclusion, P. multocida, M. haemolytica, H. somni and M. bovis are the important opportunistic pathogens of respiratory tract in cattle and these pathogens have a major role during infections. But multifactorial nature of bovine respiratory disease and immune system affected the formation of the disease. Hence, firstly cattle's immunity should be strengthened and other conditions should be kept under control.