Helicobacter turcicus sp. nov., a catalase- negative new member of the Helicobacter genus, isolated from Anatolian Ground Squirrel (Spermophilus xanthoprymnus) in Turkey


AYDIN F., KARAKAYA E., Kayman T., ABAY S., SATICIOĞLU İ. B.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol.72, no.5, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 72 Issue: 5
  • Publication Date: 2022
  • Doi Number: 10.1099/ijsem.0.005338
  • Journal Name: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Keywords: Anatolian ground squirrel, Helicobacter, feces, novel species, overall genome-related index, GASTRIC HELICOBACTERS, MINIMAL STANDARDS, CAMPYLOBACTER, IDENTIFICATION, PULLORUM, SEQUENCES, ANIMALS, PYLORI, TESTS, TREES
  • Erciyes University Affiliated: Yes

Abstract

Eleven Gram-negative, curved and S-shaped, oxidase activity positive, catalase activity negative bacterial isolates recovered from faeces of Anatolian ground squirrel (Spermophilus xanthoprymnus) in the city of Kayseri, Turkey, were subjected to a polyphasic taxonomic study. Results of a genus-specific PCR revealed that these isolates belonged to the genus Helicobacter. The 16S rRNA gene sequence analysis revealed that the 11 isolates had over 99 % sequence identity with each other and were most closely related to Helicobacter ganmani CMRI H02(T) with 97.0-97.1 % identity levels and they formed a novel phylogenetic line within the genus Helicobacter. Faydin-H64 and Faydin-H70(T) strains were subjected to gyrA and atpA gene and whole genome sequence analyses. These two Helicobacter strains formed separate phylogenetic clades, divergent from other known Helicobacter species. The DNA G+C content and genome size of the strain Faydin-H70(T) were 35.3 mol% and 1.7 Mb, respectively. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain Faydin-H70(T) and its close phylogenetic neighbour H. winghamensis ATCC BAA-430(T) were determined as 81.7 and 34.9%, respectively. Pairwise sequence comparison showed that it was closely related to H. ganmani CMRI H02(T) however it shared the highest ANI and dDDH values with H. winghamensis ATCC BAA-430(T). The data obtained from the polyphasic taxonomy approach, including phenotypic characterization and whole-genome sequences, revealed that these strains represent a novel species within the genus Helicobacter, for which the name Helicobacter turcicus sp. nov., is proposed with Faydin-H70T as the type strain (=DSM 112556(T)=LMG 32335(T)).