The goal of this research aimed to develop an accurate and reproducible textile-based optical fiber sensor for automotive seat occupancy. In our previous publication, the response of perfluorinated (PF) graded index (GI) polymer optical fibers (POFs) (62.5/750 and 62.5/490m) to bending and tensile loading was investigated. In this study, the response of the PF GI POFs to cyclic loading was investigated. The repeated loading and unloading the POF sensor would experience due to car vibrations and multiple uses by seat occupants, might cause fatigue failure to the POF sensor. The results showed that the Cytop-1 did not show any permanent deformation up to 500 cycles at strain rates 4 and 60mm/min at a gage length of 76.2mm in its elastic sensitive strain region. The Cytop-2 showed permanent deformation at 3.5% strain after 500 cycles at a gage length of 76.2mm. Thus, the Cytop-1 was found out to be more appropriate to be used as an optical fiber sensor for automotive seat occupancy sensing relative to the Cytop-2. In this study, a theoretical approach of the behavior of PF GI POF to cyclic loading was also provided.