The protective effects of Anakinra in a neonatal rat model of necrotizing enterocolitis

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Gunes H., Ipek S., Yurttutan S., Kuloglu T., Tolun F., Kazanci U., ...Daha Fazla

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.51, sa.5, ss.2727-2733, 2021 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3906/sag-2103-275
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.2727-2733
  • Anahtar Kelimeler: Anakinra, apoptosis, caspase-3, interleukin 18, necrotizing enterocolitis, rat, RECEPTOR ANTAGONIST, INTESTINAL DAMAGE, N-ACETYLCYSTEINE, ISCHEMIA, INJURY, INTERLEUKIN-1, APOPTOSIS, SEVERITY, NECROSIS
  • Erciyes Üniversitesi Adresli: Hayır

Özet

Background/aim: Necrotizing enterocolitis (NEC) is a commonly seen life-threatening condition in newborns characterized by ischemic necrosis. This study aimed to investigate anakinras effects, an interleukin-1 receptor antagonist, on oxidative stress, inflammation, and tissue necrosis in an NEC rat model. Materials and methods: Forty Wistar albino pups were divided into four groups randomly as follows; group 1, control group; group 2, anakinra-treated control group; group 3, NEC group; and group 4, NEC and anakinra treatment group. The rats were given hyperosmolar formula feeding, and they were exposed to hypoxia after cold stress at +4 degrees C and oxygen in order to create the NEC model. On the 4th day of the experiment, the pups were decapitated, and the intestinal tissues were resected for biochemical and histopathologic examination. Results: Microscopic injury scores and apoptotic indexes were higher in group 3 than the control group (p < 0.001, p = 0.002, respectively), and there was a significant decrease after anakinra. Interleukin 18 and caspase-3 levels increased with NEC and decreased significantly after administration of anakinra (p = 0.006, p = 0.004, respectively). Malondialdehyde and glutathione peroxidase levels also increased compared with the control group (p = 0.019, p = 0.002, respectively). Conclusion: In this experimental study, we found that anakinra had antiinflammatory and antioxidant effects and was protective against intestinal injury and apoptosis.