The Effect of Cyclosporine A on Proteins Controlling Intracellular Calcium Concentration in Breast Cancer Cells

CANER A., ÖNAL M. G., BİTGEN N., SEZER G.

JOURNAL OF MEMBRANE BIOLOGY, cilt.255, sa.1, ss.33-39, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 255 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s00232-021-00201-1
  • Dergi Adı: JOURNAL OF MEMBRANE BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Chemical Abstracts Core, CINAHL, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.33-39
  • Anahtar Kelimeler: Breast cancer, Cyclosporin A, Calcineurin, cMyc, PMCA, TUMOR-CELLS, CALCINEURIN, CALMODULIN, GROWTH, DEATH, PROLIFERATION, PROGRESSION, ACTIVATION, NFATC1, MYC
  • Erciyes Üniversitesi Adresli: Evet

Özet

Cyclosporine A (CsA) is an immunosuppressive drug commonly used to prevent autoimmune diseases. At the same time, CsA is a calcineurin (CaN) inhibitor. It affects the intracellular calcium signaling pathway. The effect of CsA on breast cancer cells, MDA-MB-231, plasma membrane calcium pump 1 (PMCA1), calmodulin (CaM), calcineurin (CaN), and cMyc, which are proteins that affect calcium signaling, were investigated. CsA inhibited the proliferation of MDA-MB-231 cells but did not affect the migration of the cells. After 24 h of incubation, CsA suppressed the PMCA1 protein, which pumps intracellular calcium out of the cell. At the same time, calcium started to accumulate inside the cell and CaM protein was expressed, while PMCA1 was suppressed. The CaN protein was suppressed 72 h after the administration of CsA, but the cMyc protein was expressed. Interestingly, 24 h incubation when the PMCA1 protein is down-regulated after the duration of time, the cMyc protein is also down-regulated. Although the indirect effect of CaN and cMyc is known, this relationship between PMCA1 and cMyc was not known. As a result, it has been shown that CsA affects the PMCA pump by disrupting the intracellular calcium pathway in breast cancer cells.