The objective of this project is to (a) assess the risk of invasive mosquito species and arboviruses to human health and (b) develop control strategies and an integrated mosquito management program (IMMP) to prevent vector proliferation and transmission of mosquito-borne arboviruses in Georgia, Ukraine, and Turkey (referred to as Black Sea region). Using the framework of the One-Health concept, this project will combine field and laboratory techniques (including molecular diagnostics, serology, sequencing, and phylogenetic analysis) to characterize potential vector populations, distributions and study the epidemiology and prevalence of diseases caused by dengue virus (DENV), Zika virus (ZIKV), chikungunya virus (CHIKV), and West Nile virus (WNV) in human populations. Arbovirus surveillance and genome sequencing will allow for the identification of other emerging arboviruses. A One-Health approach relies on the cutting-edge of science to advance human health through the recognition of interconnections between health and the environment (in a broad sense) and using novel tools and methodologies among multiple disciplines. Our goal is to improve international collaborations, through research and training workshops, between the U.S. and countries of the Black Sea region for long-term sustainment of surveillance and control of mosquito borne arboviruses.