COMPARISON OF QCM DETECTION SYSTEMS FROM GENETIC INSPECTION


Thesis Type: Postgraduate

Institution Of The Thesis: Erciyes Üniversitesi, Mühendislik Fakültesi, Biyomedikal Mühendisliği , Turkey

Approval Date: 2018

Thesis Language: Turkish

Student: Zeynep GÜNAYDIN

Supervisor: Mehmet Çağrı Soylu

Abstract:

Early diagnosis of the disease plays a very important role in the treatment. Today, the Polymerase Chain Reaction (PCR) is a method used genetically to diagnose diseases. Methods that can be used in place of PCR are being investigated. Because the PCR process is long, the investment costs are expensive and only fully developed laboratories, which can only be accessed and developed by developed countries, are a diagnostic method. A different method of detecting genetic susceptibility through saliva has a lower risk of infection and may be preferred because the patient is not invited. The risk of infecting health personnel and other infectious diseases that provide treatment is much less in saliva than in blood samples. In this study, two different Quartz Crystal Microbalance (QCM) devices were used. The sensor surfaces of the devices were exposed to the (3-Mercaptopropyl) trimethoxysilane (MPS) coating agent to increase the stability of the sensor and provide a suitable surface for adequate immobilization. Synthetic probe DNA (pDNA) is placed on the sensor surface to detect synthetic target DNA (tDNA). The devices were compared under the same conditions with the same experiments. The results have been evaluated by considering several parameters. Significantly comparable results have been obtained. The results also show that the genetic material in the model saliva is detected without the need for isolation, purification and amplification.https://tez.yok.gov.tr/UlusalTezMerkezi/TezGoster?key=fS4sqEZr79C_n60Rk6MjFcg4G5Zgbu81WEzuTdmhiu7kouPXuCskdCieTL5nRUj8